Effects of extraction technology on bioactivities from polysaccharide-richGanoderma lucidum

In this study, the polysaccharide-riched extract from Ganoderma lucidum was obtained by different extraction methods including hot water (HWE), ultrasonic-assisted (UHWE), enzyme-assisted (EAE), and enzyme (UAEE). The total polysaccharide content in UAEE (3.721±0.134%) higher than HWE (1.783±0.156%), UHWE (1.886±0.148%), EAE (2.133±0.139%). G. then evaluated for antioxidant activity 2,2-diphenyl-1-1picrylhydrazyl (DPPH) ferric reducing power (FRAP) methods. antibacterial assessed via half-maximal inhibitory concentration (IC50). DPPH radical scavenging of 42.334%, while result FRAP shows much better HWE, UHWE, Furthermore, extracted showed against Bacillus cereus, subtilis, Staphylococcus aureus with IC50 range 0.069-0.096 g/ml.